Fat embolism and sperm whale ship strikes. Submitted to Front. Mar. Sci.
Marina Arregui, Yara Bernaldo de QuirÃ³s, Pedro Saavedra, Eva Sierra, Cristian M. SuÃ¡rez-Santana, Manuel Arbelo, JosuÃ© DÃaz-Delgado, Raquel Puig-Lozano, Marisa Andrada and Antonio FernÃ¡ndez
Strikes between vessels and cetaceans have significantly increased worldwide in the last decades. The Canary archipelago is a geographical area with an important overlap of high cetacean diversity and maritime traffic, including high-speed ferries. Sperm whales (Physeter macrocephalus), currently listed as a vulnerable species, are severely impacted by ship strikes. Nearly 60% of sperm whalesâ€™ deaths are due to ship strikes in the Canary Islands. In such cases, subcutaneous, muscular and visceral extensive hemorrhages and hematomas, indicate unequivocal antemortem trauma. However, when carcasses are highly autolysed, it is challenging to distinguish whether the trauma occured ante- or postmortem. The presence of fat emboli within the lung microvasculature is used to determine a severe â€œin vivoâ€ trauma in other species. We hypothesized fat emboli detection could be a feasible, reliable and accurate forensic tool to determine ante-mortem ship strikes in stranded sperm whales, even in decomposed carcasses. In this study, we evaluated the presence of fat emboli by an osmium tetroxide (OsO4) based histochemical techniquein lung tissue of 24 sperm whales, 16 of them with evidence of ship strike, stranded and necropsied in the Canaries between 2000 and 2017. About 70% of them presented an advanced autolysis. Histological examination revealed the presence of OsO4-positive fat emboli in 13 out of the 16 sperm whales with signs of ship strike with varying degrees of abundance and distribution. A predictive model for strikes was developed based on the area ocuppied by fat emboli and the age-class of the animals. The results demonstrated: 1) the usefulness of fat detection as a diagnostic tool for â€œin vivoâ€ trauma, even in decomposed tissues kept in formaldehyde for long periods of time; and 2) that, during this 18-year period, at least, 81% of the sperm whales with signs of ship strike were alive at the moment of the strike and died subsequently. This information is highly valuable in order to implement proper mitigation measures in this area.